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NDUFB6 Rabbit pAb

Anti-NDUFB6 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S218060

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S218060
Product Name:   Anti-NDUFB6 Rabbit Polyclonal Antibody
Synonyms:   CI; B17
UNIPROT ID:   O95139 (Gene Accession – BC009801 )
Background:    The protein encoded by this gene is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Alternative splicing occurs at this locus and three transcript variants encoding distinct isoforms have been identified.
Immunogen:    Full length fusion protein
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism, Cancer, Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human prostate cancer tissue using 218060(NDUFB6 Antibody) at a dilution of 1/20(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human prostate cancer tissue is first treated with the fusion protein and then with 218060(Anti-NDUFB6 Antibody) at dilution 1/20.

The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using 218060(Anti-NDUFB6 Antibody) at a dilution of 1/20.

In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with fusion protein and then with D223633(Anti-NDUFB6 Antibody) at dilution 1/20.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-6: 293T, Hela, PC-3, Lncap and 231 cell, Human fetal muscle tissue;
Primary antibody: 218060(NDUFB6 Antibody) at dilution 1/500;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 second
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