Email: info@neweastbio.com   |  Call: 6109452007

NECTIN4 Rabbit pAb

Anti-NECTIN4 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217644

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217644
Product Name:   Anti-NECTIN4 Rabbit Polyclonal Antibody
Synonyms:   LNIR; PRR4; EDSS1; PVRL4; nectin-4
UNIPROT ID:   Q96NY8 (Gene Accession – BC010423 )
Background:    This gene encodes a member of the nectin family. The encoded protein contains two immunoglobulin-like (Ig-like) C2-type domains and one Ig-like V-type domain. It is involved in cell adhesion through trans-homophilic and -heterophilic interactions. It is a single-pass type I membrane protein. The soluble form is produced by proteolytic cleavage at the cell surface by the metalloproteinase ADAM17/TACE. The secreted form is found in both breast tumor cell lines and breast tumor patients. Mutations in this gene are the cause of ectodermal dysplasia-syndactyly syndrome type 1, an autosomal recessive disorder. Alternatively spliced transcript variants have been found but the full-length nature of the variant has not been determined.
Immunogen:    Fusion protein of human NECTIN4
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human esophagus cancer tissue using 217644(NECTIN4 Antibody) at a dilution of 1/60(Secreted).

In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with the fusion protein and then with 217644(Anti-NECTIN4 Antibody) at dilution 1/60.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: SKOV3 cell lysate;
Primary antibody: 217644(NECTIN4 Antibody) at dilution 1/550;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 30 seconds
          Publications