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NEFM Rabbit pAb

Anti-NEFM Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S220744

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S220744
Product Name:   Anti-NEFM Rabbit Polyclonal Antibody
Synonyms:   NFM; NEF3; NF-M
UNIPROT ID:   P07197 (Gene Accession – NP_005373 )
Background:    Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Immunogen:    Synthetic peptide of human NEFM
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-100;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Neuroscience
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human cervical cancer tissue using 220744(NEFM Antibody) at a dilution of 1/50(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with the synthetic peptide and then with 220744(Anti-NEFM Antibody) at dilution 1/50.

The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 220744(Anti-NEFM Antibody) at a dilution of 1/50.

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with synthetic peptide and then with D261950(Anti-NEFM Antibody) at dilution 1/50.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: Mouse fetal brain tissue, Mouse brain tissue, Human cerebella tissue lysates;
Primary antibody: 220744(NEFM Antibody) at dilution 1/800;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 5 seconds
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