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NFKBIE Rabbit pAb

Anti-NFKBIE Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216287

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216287
Product Name:   Anti-NFKBIE Rabbit Polyclonal Antibody
Synonyms:   IKBE
UNIPROT ID:   O00221 (Gene Accession – BC011676 )
Background:    The protein encoded by this gene binds to components of NF-kappa-B, trapping the complex in the cytoplasm and preventing it from activating genes in the nucleus. Phosphorylation of the encoded protein targets it for destruction by the ubiquitin pathway, which activates NF-kappa-B by making it available to translocate to the nucleus. NFKBIE protein expression is up-regulated following NF-κB activation and during myelopoiesis. NFKBIE is able to inhibit NF-κB-directed transactivation via cytoplasmic retention of REL proteins. NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NF-κB transcription factor complex. The NF-κB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm.
Immunogen:    Fusion protein of human NFKBIE
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 200-1000;ELISA: 1000-2000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Epigenetics and Nuclear Signaling, Immunology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human lymphoma tissue using 216287(NFKBIE Antibody) at a dilution of 1/25(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human lymphoma tissue is first treated with the fusion protein and then with 216287(Anti-NFKBIE Antibody) at dilution 1/25.

The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using 216287(Anti-NFKBIE Antibody) at a dilution of 1/25.

In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with fusion protein and then with D220137(Anti-NFKBIE Antibody) at dilution 1/25.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane: PC3 cells;
Primary antibody: 216287(NFKBIE Antibody) at dilution 1/250;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 30 minutes
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