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NIT2 Rabbit pAb

Anti-NIT2 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S220143

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S220143
Product Name:   Anti-NIT2 Rabbit Polyclonal Antibody
Synonyms:   
UNIPROT ID:   Q9NQR4 (Gene Accession – NP_064587 )
Background:    Has a omega-amidase activity. The role of omega-amidase is to remove potentially toxic intermediates by converting alpha-ketoglutaramate and alpha-ketosuccinamate to biologically useful alpha-ketoglutarate and oxaloacetate, respectively. Overexpression decreases the colony-forming capacity of cultured cells by arresting cells in the G2 phase of the cell cycle.
Immunogen:    Synthetic peptide of human NIT2
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 1000-5000;ELISA: 2000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human colon cancer tissue using 220143(NIT2 Antibody) at a dilution of 1/40(Cytoplasm, Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human colon cancer tissue is first treated with the synthetic peptide and then with 220143(Anti-NIT2 Antibody) at dilution 1/40.

The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using 220143(Anti-NIT2 Antibody) at a dilution of 1/40.

In comparision with the IHC on the left, the same paraffin-embedded Human ovarian cancer tissue is first treated with synthetic peptide and then with D261032(Anti-NIT2 Antibody) at dilution 1/40.

Gel: 10%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: Mouse liver tissue, Mouse kidney tissue, RAW264.7 cells;
Primary antibody: 220143(NIT2 Antibody) at dilution 1/950;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 90 seconds
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