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NMT1 Rabbit pAb

Anti-NMT1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S220753

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S220753
Product Name:   Anti-NMT1 Rabbit Polyclonal Antibody
Synonyms:   NMT
UNIPROT ID:   P30419 (Gene Accession – NP_066565 )
Background:    Myristate, a rare 14-carbon saturated fatty acid, is cotranslationally attached by an amide linkage to the N-terminal glycine residue of cellular and viral proteins with diverse functions. N-myristoyltransferase catalyzes the transfer of myristate from CoA to proteins. N-myristoylation appears to be irreversible and is required for full expression of the biologic activities of several N-myristoylated proteins, including the alpha subunit of the signal-transducing guanine nucleotide-binding protein (G protein).
Immunogen:    Synthetic peptide of human NMT1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 200-1000;ELISA: 1000-2000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cancer, Stem Cells
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human ovarian cancer tissue using 220753(NMT1 Antibody) at a dilution of 1/45(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human ovarian cancer tissue is first treated with the synthetic peptide and then with 220753(Anti-NMT1 Antibody) at dilution 1/45.

The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using 220753(Anti-NMT1 Antibody) at a dilution of 1/45.

In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with synthetic peptide and then with D261963(Anti-NMT1 Antibody) at dilution 1/45.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: Human kidney tissue;
Primary antibody: 220753(NMT1 Antibody) at dilution 1/200;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 3 minutes
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