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NOX5 Rabbit pAb

Anti-NOX5 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217654

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217654
Product Name:   Anti-NOX5 Rabbit Polyclonal Antibody
Synonyms:   
UNIPROT ID:   Q96PH1 (Gene Accession – BC125098 )
Background:    This gene is predominantly expressed in the testis and lymphocyte-rich areas of spleen and lymph nodes. It encodes a calcium-dependen NADPH oxidase that generates superoxide, and functions as a calcium-dependent proton channel that may regulate redox-dependent processes in lymphocytes and spermatozoa. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Immunogen:    Fusion protein of human NOX5
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism, Cancer, Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 217654(NOX5 Antibody) at a dilution of 1/60(Cell membrane).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the fusion protein and then with 217654(Anti-NOX5 Antibody) at dilution 1/60.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-6: PC-3, Raji, SKOV3, Jurkat, Hela, 293T cell lysates;
Primary antibody: 217654(NOX5 Antibody) at dilution 1/850;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 1 minute
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