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NRAS Rabbit pAb

Anti-NRAS Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S222351

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S222351
Product Name:   Anti-NRAS Rabbit Polyclonal Antibody
Synonyms:   NS6; CMNS; NCMS; ALPS4; N-ras; NRAS1
UNIPROT ID:   P01111 (Gene Accession – NP_002515. )
Background:    This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia.
Immunogen:    Synthetic peptide of human NRAS
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-100;WB: 200-1000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human breast cancer tissue using 222351(NRAS Antibody) at a dilution of 1/50(Cytoplasm and Cell membrane).

In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with the synthetic peptide and then with 222351(Anti-NRAS Antibody) at dilution 1/50.

The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using 222351(Anti-NRAS Antibody) at a dilution of 1/50.

In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with synthetic peptide and then with D264460(Anti-NRAS Antibody) at dilution 1/50.

Gel: 10%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Human cerebella tissue, Mouse brain tissue lysates;
Primary antibody: 222351(NRAS Antibody) at dilution 1/250;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 1 minute
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