Email: info@neweastbio.com   |  Call: 6109452007

NUAK1 Rabbit pAb

Anti-NUAK1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S220305

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S220305
Product Name:   Anti-NUAK1 Rabbit Polyclonal Antibody
Synonyms:   ARK5
UNIPROT ID:   O60285 (Gene Accession – NP_055655 )
Background:    NUAK family SNF1-like kinase 1 also known as AMPK-related protein kinase 5 (ARK5) is an enzyme that in humans is encoded by the NUAK1 gene. Serine/threonine-protein kinase involved in various processes such as cell adhesion, regulation of cell ploidy and senescence, cell proliferation and tumor progression. Phosphorylates ATM, CASP6, LATS1, PPP1R12A and p53/TP53. Acts as a regulator of cellular senescence and cellular ploidy by mediating phosphorylation of ‘Ser-464’ of LATS1, thereby controlling its stability. Controls cell adhesion by regulating activity of the myosin protein phosphatase 1 (PP1) complex. Acts by mediating phosphorylation of PPP1R12A subunit of myosin PP1: phosphorylated PPP1R12A then interacts with 14-3-3, leading to reduced dephosphorylation of myosin MLC2 by myosin PP1.
Immunogen:    Synthetic peptide of human NUAK1
Applications:    ELISA, IHC
Recommended Dilutions:    IHC: 100-300; ELISA: 2000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human lung cancer tissue using 220305(NUAK1 Antibody) at a dilution of 1/70(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human lung cancer tissue is first treated with the synthetic peptide and then with 220305(Anti-NUAK1 Antibody) at dilution 1/70.

The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 220305(Anti-NUAK1 Antibody) at a dilution of 1/70.

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with synthetic peptide and then with D261308(Anti-NUAK1 Antibody) at dilution 1/70.
          Publications