Email: info@neweastbio.com   |  Call: 6109452007

ORC4 Rabbit pAb

Anti-ORC4 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S218846

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S218846
Product Name:   Anti-ORC4 Rabbit Polyclonal Antibody
Synonyms:   ORC4L; ORC4P
UNIPROT ID:   O43929 (Gene Accession – BC005388 )
Background:    The origin recognition complex (ORC) is a highly conserved six subunit protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. This gene encodes a subunit of the ORC complex. Several alternatively spliced transcript variants, some of which encode the same protein, have been reported for this gene.
Immunogen:    Fusion protein of human ORC4
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-300;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human esophagus cancer tissue using 218846(ORC4 Antibody) at a dilution of 1/80(Nucleus and Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with the fusion protein and then with 218846(Anti-ORC4 Antibody) at dilution 1/80.

The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using 218846(Anti-ORC4 Antibody) at a dilution of 1/80.

In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with fusion protein and then with D225358(Anti-ORC4 Antibody) at dilution 1/80.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: HepG2 and Hela cell lysates;
Primary antibody: 218846(ORC4 Antibody) at dilution 1/550;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 10 seconds
          Publications