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PAWR Rabbit pAb

Anti-PAWR Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S213706

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S213706
Product Name:   Anti-PAWR Rabbit Polyclonal Antibody
Synonyms:   PAR4, Par-4
UNIPROT ID:   Q96IZ0 (Gene Accession – NP_002574 )
Background:    The tumor suppressor WT1 represses and activates transcription. The protein encoded by this gene is a WT1-interacting protein that itself functions as a transcriptional repressor. It contains a putative leucine zipper domain which interacts with the zinc finger DNA binding domain of WT1. This protein is specifically upregulated during apoptosis of prostate cells.
Immunogen:    Synthetic peptide of human PAWR
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 1000-3000;ELISA: 1000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling, Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human colon cancer tissue using 213706(PAWR Antibody) at a dilution of 1/60(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human colon cancer tissue is first treated with the synthetic peptide and then with 213706(Anti-PAWR Antibody) at dilution 1/60.

The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using 213706(Anti-PAWR Antibody) at a dilution of 1/60.

In comparision with the IHC on the left, the same paraffin-embedded Human ovarian cancer tissue is first treated with synthetic peptide and then with D160694(Anti-PAWR Antibody) at dilution 1/60.

Gel: 10%SDS-PAGE, Lysate: 30 μg;
Lane 1-2: Hela cells, A549 cells;
Primary antibody: 213706(PAWR Antibody) at dilution 1/1200;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 10 seconds
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