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PCBD1 Rabbit pAb

Anti-PCBD1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S219182

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S219182
Product Name:   Anti-PCBD1 Rabbit Polyclonal Antibody
Synonyms:   PCD; PHS; DCOH; PCBD
UNIPROT ID:   P61457 (Gene Accession – BC006324 )
Background:    This gene encodes a member of the pterin-4-alpha-carbinolamine dehydratase family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The encoded protein functions as both a dehydratase involved in tetrahydrobiopterin biosynthesis, and as a cofactor for HNF1A-dependent transcription. A deficiency of this enzyme leads to hyperphenylalaninemia. Alternative splicing results in multiple transcript variants.
Immunogen:    Fusion protein of human PCBD1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-300;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism, Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human breast cancer tissue using 219182(PCBD1 Antibody) at a dilution of 1/60(Cytoplasm and Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with the fusion protein and then with 219182(Anti-PCBD1 Antibody) at dilution 1/60.

The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 219182(Anti-PCBD1 Antibody) at a dilution of 1/60.

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with fusion protein and then with D226009(Anti-PCBD1 Antibody) at dilution 1/60.

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane: Mouse kidney tissue lysate;
Primary antibody: 219182(PCBD1 Antibody) at dilution 1/800;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 1 minute
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