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PCK2 Rabbit pAb

Anti-PCK2 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217605

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217605
Product Name:   Anti-PCK2 Rabbit Polyclonal Antibody
Synonyms:   PEPCK; PEPCK2; PEPCK-M
UNIPROT ID:   Q16822 (Gene Accession – BC001454 )
Background:    This gene encodes a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of guanosine triphosphate (GTP). A cytosolic form of this protein is encoded by a different gene and is the key enzyme of gluconeogenesis in the liver. Alternatively spliced transcript variants have been described.
Immunogen:    Fusion protein of human PCK2
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 100-300;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism, Signal Transduction, Cancer, Cardiovascular
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 217605(PCK2 Antibody) at a dilution of 1/60(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the fusion protein and then with 217605(Anti-PCK2 Antibody) at dilution 1/60.

The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using 217605(Anti-PCK2 Antibody) at a dilution of 1/60.

In comparision with the IHC on the left, the same paraffin-embedded Human colon cancer tissue is first treated with fusion protein and then with D222686(Anti-PCK2 Antibody) at dilution 1/60.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: 293T cells, Jurkat cells, Hela cells;
Primary antibody: 217605(PCK2 Antibody) at dilution 1/750;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 10 minutes
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