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PCMT1 Rabbit pAb

Anti-PCMT1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217674

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217674
Product Name:   Anti-PCMT1 Rabbit Polyclonal Antibody
Synonyms:   PIMT
UNIPROT ID:   P22061 (Gene Accession – BC007501 )
Background:    This gene encodes a member of the type II class of protein carboxyl methyltransferase enzymes. The encoded enzyme plays a role in protein repair by recognizing and converting D-aspartyl and L-isoaspartyl residues resulting from spontaneous deamidation back to the normal L-aspartyl form. The encoded protein may play a protective role in the pathogenesis of Alzheimer’s disease, and single nucleotide polymorphisms in this gene have been associated with spina bifida and premature ovarian failure. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Oct 2011]
Immunogen:    Fusion protein of human PCMT1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 200-300;WB: 1000-5000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human esophagus cancer tissue using 217674(PCMT1 Antibody) at a dilution of 1/160(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with the fusion protein and then with 217674(Anti-PCMT1 Antibody) at dilution 1/160.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-6: Jurkat, Raji, 231, HepG2, Hela and 293T cell lysates;
Primary antibody: 217674(PCMT1 Antibody) at dilution 1/1000;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 1 minute
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