Email: info@neweastbio.com   |  Call: 6109452007

PCNA Rabbit pAb

Anti-PCNA Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216220

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216220
Product Name:   Anti-PCNA Rabbit Polyclonal Antibody
Synonyms:   ATLD2
UNIPROT ID:   P12004 (Gene Accession – BC000491 )
Background:    The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.
Immunogen:    Fusion protein of human PCNA
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling, Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin-embedded Human colorectal cancer tissue using 216220(PCNA Antibody) at a dilution of 1/35(Nucleus).

Gel: 12%SDS-PAGE, Lysate: 40 μg;
Lane 1-4: 293T, K562, Jurkat, RAW264.7 cell lysates;
Primary antibody: 216220(PCNA Antibody) at dilution 1/400;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 3 seconds
          Publications