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PDE4A Rabbit pAb

Anti-PDE4A Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S220790

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S220790
Product Name:   Anti-PDE4A Rabbit Polyclonal Antibody
Synonyms:   PDE4; DPDE2; PDE46
UNIPROT ID:   P27815 (Gene Accession – NP_001104777 )
Background:    The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE4 subfamily. This PDE hydrolyzes the second messenger, cAMP, which is a regulator and mediator of a number of cellular responses to extracellular signals. Thus, by regulating the cellular concentration of cAMP, this protein plays a key role in many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Immunogen:    Synthetic peptide of human PDE4A
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human thyroid cancer tissue using 220790(PDE4A Antibody) at a dilution of 1/40(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with the synthetic peptide and then with 220790(Anti-PDE4A Antibody) at dilution 1/40.

The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using 220790(Anti-PDE4A Antibody) at a dilution of 1/40.

In comparision with the IHC on the left, the same paraffin-embedded Human brain tissue is first treated with synthetic peptide and then with D262029(Anti-PDE4A Antibody) at dilution 1/40.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Hela cells, A172 cells;
Primary antibody: 220790(PDE4A Antibody) at dilution 1/300;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 20 seconds
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