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PDK2 Rabbit pAb

Anti-PDK2 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S213711

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S213711
Product Name:   Anti-PDK2 Rabbit Polyclonal Antibody
Synonyms:   PDHK2
UNIPROT ID:   Q15119 (Gene Accession – NP_002602 )
Background:    This gene encodes a member of the pyruvate dehydrogenase kinase family. The encoded protein phosphorylates pyruvate dehydrogenase, down-regulating the activity of the mitochondrial pyruvate dehydrogenase complex. Overexpression of this gene may play a role in both cancer and diabetes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Immunogen:    Synthetic peptide of human PDK2
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 200-1000;ELISA: 1000-2000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism, Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 213711(PDK2 Antibody) at a dilution of 1/40(Mitochondrion matri,x Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 213711(Anti-PDK2 Antibody) at dilution 1/40.

The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using 213711(Anti-PDK2 Antibody) at a dilution of 1/40.

In comparision with the IHC on the left, the same paraffin-embedded Human lung cancer tissue is first treated with synthetic peptide and then with D160702(Anti-PDK2 Antibody) at dilution 1/40.

Gel: 10%SDS-PAGE, Lysate: 50 μg;
Lane: Mouse heart tissue;
Primary antibody: 213711(PDK2 Antibody) at dilution 1/500;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 minute
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