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PDX1 Rabbit pAb

Anti-PDX1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S219969

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S219969
Product Name:   Anti-PDX1 Rabbit Polyclonal Antibody
Synonyms:   GSF, IPF1, IUF1, IDX-1, MODY4, PDX-1, STF-1
UNIPROT ID:   P52945 (Gene Accession – NP_000200 )
Background:    The protein encoded by this gene is a transcriptional activator of several genes, including insulin, somatostatin, glucokinase, islet amyloid polypeptide, and glucose transporter type 2. The encoded nuclear protein is involved in the early development of the pancreas and plays a major role in glucose-dependent regulation of insulin gene expression. Defects in this gene are a cause of pancreatic agenesis, which can lead to early-onset insulin-dependent diabetes mellitus (NIDDM), as well as maturity onset diabetes of the young type 4 (MODY4).
Immunogen:    Synthetic peptide of human PDX1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 15-50;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Epigenetics and Nuclear Signaling, Cancer, Metabolism, Cardiovascular, Developmental Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human colon cancer tissue using 219969(PDX1 Antibody) at a dilution of 1/30(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human colon cancer tissue is first treated with the synthetic peptide and then with 219969(Anti-PDX1 Antibody) at dilution 1/30.

The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using 219969(Anti-PDX1 Antibody) at a dilution of 1/30.

In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with synthetic peptide and then with D260705(Anti-PDX1 Antibody) at dilution 1/30.

Gel: 10%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: Hela cells, Jurkat cells;
Primary antibody: 219969(PDX1 Antibody) at dilution 1/450;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 minute
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