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PDXK Rabbit pAb

Anti-PDXK Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S219321

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S219321
Product Name:   Anti-PDXK Rabbit Polyclonal Antibody
Synonyms:   PKH; PNK; PRED79; C21orf97; HEL-S-1a; C21orf124
UNIPROT ID:   O00764 (Gene Accession – BC000123 )
Background:    The protein encoded by this gene phosphorylates vitamin B6, a step required for the conversion of vitamin B6 to pyridoxal-5-phosphate, an important cofactor in intermediary metabolism. The encoded protein is cytoplasmic and probably acts as a homodimer. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.
Immunogen:    Fusion protein of human PDXK
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 1000-5000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism, Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 219321(PDXK Antibody) at a dilution of 1/75(Cytoplasm and Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the fusion protein and then with 219321(Anti-PDXK Antibody) at dilution 1/75.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: Rat liver tissue, MCF-7 cell, Mouse brain tissue lysates;
Primary antibody: 219321(PDXK Antibody) at dilution 1/2000;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 20 seconds
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