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PFKFB4 Rabbit pAb

Anti-PFKFB4 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S214142

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S214142
Product Name:   Anti-PFKFB4 Rabbit Polyclonal Antibody
Synonyms:   
UNIPROT ID:   Q16877 (Gene Accession – NP_004558 )
Background:    The protein encoded by this gene is one of four bifunctional kinase/phosphatases that regulate the concentration of the glycolytic byproduct fructose-2,6-bisphosphate (F2,6BP). The encoded protein is highly expressed in cancer cells and is induced by hypoxia. This protein is essential to the survival of cancer cells under conditions of hypoxia, because it increases the amount of F2,6BP and ATP at a time when the cell cannot produce much of them. This finding suggests that this protein may be a good target for disruption in cancer cells, hopefully imperiling their survival. Several transcript variants encoding different isoforms have been found for this gene.
Immunogen:    Synthetic peptide of human PFKFB4
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 200-1000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism, Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using 214142(PFKFB4 Antibody) at a dilution of 1/35(Cytoplasm).

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: Raji, 231, 293T cell lysates;
Primary antibody: 214142(PFKFB4 Antibody) at dilution 1/200;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 1 minute
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