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PFKM Rabbit pAb

Anti-PFKM Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S218780

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S218780
Product Name:   Anti-PFKM Rabbit Polyclonal Antibody
Synonyms:   GSD7; PFK1; PFKA; PFKX; PFK-1; ATP-PFK; PPP1R122
UNIPROT ID:   P08237 (Gene Accession – BC021203 )
Background:    Three phosphofructokinase isozymes exist in humans: muscle, liver and platelet. These isozymes function as subunits of the mammalian tetramer phosphofructokinase, which catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate. Tetramer composition varies depending on tissue type. This gene encodes the muscle-type isozyme. Mutations in this gene have been associated with glycogen storage disease type VII, also known as Tarui disease. Alternatively spliced transcript variants have been described.
Immunogen:    Fusion protein of human PFKM
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-300;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism, Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human thyroid cancer tissue using 218780(PFKM Antibody) at a dilution of 1/60(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with the fusion protein and then with 218780(Anti-PFKM Antibody) at dilution 1/60.

The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using 218780(Anti-PFKM Antibody) at a dilution of 1/60.

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with fusion protein and then with D225163(Anti-PFKM Antibody) at dilution 1/60.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: Mouse brain tissue lysate;
Primary antibody: 218780(PFKM Antibody) at dilution 1/350;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 minute
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