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PGC Rabbit pAb

Anti-PGC Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216725

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216725
Product Name:   Anti-PGC Rabbit Polyclonal Antibody
Synonyms:   PEPC, PGII
UNIPROT ID:   P20142 (Gene Accession – BC042578 )
Background:    This gene encodes an aspartic proteinase that belongs to the peptidase family A1. The encoded protein is a digestive enzyme that is produced in the stomach and constitutes a major component of the gastric mucosa. This protein is also secreted into the serum. This protein is synthesized as an inactive zymogen that includes a highly basic prosegment. This enzyme is converted into its active mature form at low pH by sequential cleavage of the prosegment that is carried out by the enzyme itself. Polymorphisms in this gene are associated with susceptibility to gastric cancers. Serum levels of this enzyme are used as a biomarker for certain gastric diseases including Helicobacter pylori related gastritis. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 1.
Immunogen:    Fusion protein of human PGC
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism, Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human ovarian cancer tissue using 216725(PGC Antibody) at a dilution of 1/20(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human ovarian cancer tissue is first treated with the fusion protein and then with 216725(Anti-PGC Antibody) at dilution 1/20.

The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using 216725(Anti-PGC Antibody) at a dilution of 1/20.

In comparision with the IHC on the left, the same paraffin-embedded Human colon cancer tissue is first treated with fusion protein and then with D221129(Anti-PGC Antibody) at dilution 1/20.

Gel: 12%SDS-PAGE, Lysate: 50 μg;
Lane: 823 cells;
Primary antibody: 216725(PGC Antibody) at dilution 1/200;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 30 seconds
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