NewEast Biosciences pioneered the research and development of the antibodies for GTPases and mutated Oncogene ten years ago. GTPases involve (1) signal transduction in response to activation of cell surface receptors, including transmembrane receptors such as those mediating taste, smell and vision, (2) protein biosynthesis at the ribosome, (3) regulation of cell differentiation, proliferation, division and movement, (4) translocation of proteins through membranes, (5) transport of vesicles within the cell, and vesicle-mediated secretion and uptake, through GTPase control of vesicle coat assembly. An oncogene is a gene that has the potential to cause cancer.
We offer three unique categories of antibodies, which (1) recognize only the active configuration of GTPase (not the inactive one), (2) mutated Oncogene (not mild type) and (3) have super affinity for cAMP and cGMP (no acetylation required). We have over one thousand peer reviewed articles cited our products.
$349.00
Cat.#: N225560 |
Product Name: Anti-PHD1 Rabbit pAb |
Synonyms: Estrogen-induced tag 6; HPH-3; PHD1 |
UNIPROT ID: Q96KS0 |
Background: Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. |
Immunogen: A synthesized peptide derived from human PHD1/prolyl hydroxylase |
Applications: WB,IHC-P,ICC/IF,FC |
Recommended Dilutions: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 FC: 1/50-1/100 |
Host Species: Rabbit |
Clonality: Rabbit Polyclonal |
Clone ID: – |
MW: Calculated MW: 44 kDa; Observed MW: 44 kDa |
Isotype: IgG |
Purification: Affinity Chromatography |
Species Reactivity: Human,Mouse,Rat |
Conjugation: Unconjugated |
Modification: Unmodified |
Constituents: PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide |
Research Areas: Cardiovascular |
Storage & Shipping: Store at -20°C. Avoid repeated freezing and thawing |
Western blot analysis of PHD1 in (1) HeLa lysates; (2) A549 lysates using PHD1 antibody. |