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PHF21A Rabbit pAb

Anti-PHF21A Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S218360

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S218360
Product Name:   Anti-PHF21A Rabbit Polyclonal Antibody
Synonyms:   BHC80; BM-006
UNIPROT ID:   Q96BD5 (Gene Accession – BC015714 )
Background:    Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. PHF21A (PHD finger protein 21A), also known as BRAF35-HDAC complex protein BHC80, is a 680 amino acid nuclear protein that contains one PHD-type zinc finger and one A.T hook DNA-binding domain, suggesting involvement in transcriptional regulation events. PHF21A is a component of the BHC comple,x which is responsible for repressing transcription of neuron-specific genes in non-neuronal cells. The BHC complex acts as a chromatin modifier that deacetylates and demethylates specific sites on histones. PHF21A may act as a scaffold within the BHC complex. Predominantly expressed in brain, three isoforms of PHF21A exist as a result of alternative splicing events.
Immunogen:    Fusion protein of human PHF21A
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 40-200;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling, Neuroscience
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human prostate cancer tissue using 218360(PHF21A Antibody) at a dilution of 1/50(Cytoplasm and Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human prostate cancer tissue is first treated with the fusion protein and then with 218360(Anti-PHF21A Antibody) at dilution 1/50.

The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using 218360(Anti-PHF21A Antibody) at a dilution of 1/50.

In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with fusion protein and then with D224255(Anti-PHF21A Antibody) at dilution 1/50.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-4:A172, 293T, Hela and A375 cell lysates;
Primary antibody: 218360(PHF21A Antibody) at dilution 1/400;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 3 seconds
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