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Phospho-4E BP1 (Thr46) Rabbit mAb

Anti-Phospho-4E BP1 (Thr46) Rabbit Monoclonal Antibody
5/5

$349.00

Cat.#:  N261722

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   N261722
Product Name:   Anti-Phospho-4E BP1 (Thr46) Rabbit Monoclonal Antibody
Synonyms:   EIF4EBP1; Eukaryotic translation initiation factor 4E-binding protein 1; 4E-BP1; eIF4E-binding protein 1; Phosphorylated heat- and acid-stable protein regulated by insulin 1; PHAS-I
UNIPROT ID:   Q13541
Background:    Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation. Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity.
Immunogen:    A synthetic phosphopeptide corresponding to residues surrounding Thr45 of human eIF4EBP1
Applications:    WB,IHC-P,IP
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100 IP: 1/20
Host Species:    Rabbit
Clonality:    Rabbit Monoclonal
Clone ID:    R03-5C7
MW:    Calculated MW: 13 kDa; Observed MW: 15-20 kDa
Isotype:    IgG
Purification:    Affinity Purified
Species Reactivity:    Human,Mouse,Rat
Conjugation:    Unconjugated
Modification:    Phosphorylated
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using eIF4EBP1/eIF4EBP2/eIF4EBP3 (Phospho- T45) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Western blot analysis of Phospho-eIF4EBP1 (Thr46) in K562, C6, 3T3, Hela lysates using Phospho-4E BP1 (Thr46) antibody.
          Publications