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Phospho-Artemis (Ser516) Rabbit pAb

Anti-Phospho-Artemis (Ser516) Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  N225487

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   N225487
Product Name:   Anti-Phospho-Artemis (Ser516) Rabbit pAb
Synonyms:   SCIDA; SNM1C; A-SCID; RS-SCID; DCLREC1C
UNIPROT ID:   Q96SD1
Background:    Required for V(D)J recombination, the process by which exons encoding the antigen-binding domains of immunoglobulins and T-cell receptor proteins are assembled from individual V, (D), and J gene segments. V(D)J recombination is initiated by the lymphoid specific RAG endonuclease complex, which generates site specific DNA double strand breaks (DSBs). These DSBs present two types of DNA end structures: hairpin sealed coding ends and phosphorylated blunt signal ends. These ends are independently repaired by the non homologous end joining (NHEJ) pathway to form coding and signal joints respectively. This protein exhibits single-strand specific 5′-3′ exonuclease activity in isolation and acquires endonucleolytic activity on 5′ and 3′ hairpins and overhangs when in a complex with PRKDC. The latter activity is required specifically for the resolution of closed hairpins prior to the formation of the coding joint. May also be required for the repair of complex DSBs induced by ionizing radiation, which require substantial end-processing prior to religation by NHEJ.
Immunogen:    The antiserum was produced against synthesized peptide derived from human Artemis around the phosphorylation site of Ser516. AA range:482-531
Applications:    WB,IHC-P,ELISA
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100 ELISA: 1/10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Clone ID:   
MW:    Calculated MW: 78 kDa; Observed MW: 78 kDa
Isotype:    IgG
Purification:    Affinity Chromatography
Species Reactivity:    Human,Mouse
Conjugation:    Unconjugated
Modification:    Phosphorylated
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Western blot analysis of Phospho-Artemis (Ser516) in HepG2 cells treated with EGF , using Phospho-Artemis (Ser516) antibody.The lane on the right is blocked with the Phospho- peptide.

Immunohistochemistry analysis of paraffin-embedded Human lung carcinoma, using Phospho-Artemis (Ser516) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Sample with blocking peptide on the right.
          Publications