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Phospho-COT (Thr290) Rabbit pAb

Anti-Phospho-COT (Thr290) Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  N225513

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   N225513
Product Name:   Anti-Phospho-COT (Thr290) Rabbit pAb
Synonyms:   Mitogen-activated protein kinase kinase kinase 8; Cancer Osaka thyroid oncogene; Proto-oncogene c-Cot; Serine/threonine-protein kinase cot; Tumor progression locus 2
UNIPROT ID:   P41279
Background:    mitogen-activated protein kinase kinase kinase 8(MAP3K8) Homo sapiens This gene is an oncogene that encodes a member of the serine/threonine protein kinase family. The encoded protein localizes to the cytoplasm and can activate both the MAP kinase and JNK kinase pathways. This protein was shown to activate IkappaB kinases, and thus induce the nuclear production of NF-kappaB. This protein was also found to promote the production of TNF-alpha and IL-2 during T lymphocyte activation. This gene may also utilize a downstream in-frame translation start codon, and thus produce an isoform containing a shorter N-terminus. The shorter isoform has been shown to display weaker transforming activity. Alternate splicing results in multiple transcript variants that encode the same protein.
Immunogen:    The antiserum was produced against synthesized peptide derived from human COT around the phosphorylation site of Thr290. AA range:256-305
Applications:    WB,IHC-P,ICC/IF,ELISA
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 ELISA: 1/10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Clone ID:   
MW:    Calculated MW: 53 kDa; Observed MW: 60 kDa
Isotype:    IgG
Purification:    Affinity Chromatography
Species Reactivity:    Human,Mouse,Rat
Conjugation:    Unconjugated
Modification:    Phosphorylated
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Western blot analysis of Phospho-COT (Thr290) in 293 lysates treated with UV using Phospho-COT (Thr290) antibody. The lane on the right is blocked with the Phospho- peptide.
          Publications