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Phospho-eIF4E (Ser209) Rabbit pAb

Anti-Phospho-eIF4E (Ser209) Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  N225613

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   N225613
Product Name:   Anti-Phospho-eIF4E (Ser209) Rabbit pAb
Synonyms:   EIF4E; EIF4EL1; EIF4F; Eukaryotic translation initiation factor 4E; eIF-4E; eIF4E; eIF-4F 25 kDa subunit; mRNA cap-binding protein
UNIPROT ID:   P06730
Background:    eIF4E, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types.
Immunogen:    A synthesized peptide derived from human Phospho-eIF4E (S209)
Applications:    WB,IHC-P,ICC/IF,IP
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Clone ID:   
MW:    Calculated MW: 25 kDa; Observed MW: 25 kDa
Isotype:    IgG
Purification:    Affinity Chromatography
Species Reactivity:    Human,Mouse,Rat
Conjugation:    Unconjugated
Modification:    Phosphorylated
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Phospho-eIF4E (S209) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Western blot analysis of eIF4E (Phospho-S209) in (1) HEK293 lysates; (2) mouse spleen lysates using Phospho-eIF4E (Ser209) antibody.
          Publications