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Phospho-ErbB 2 (Tyr1221/Tyr1222) Rabbit pAb

Anti-Phospho-ErbB 2 (Tyr1221/Tyr1222) Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  N225418

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   N225418
Product Name:   Anti-Phospho-ErbB 2 (Tyr1221/Tyr1222) Rabbit pAb
Synonyms:   ERBB2; HER2; MLN19; NEU; NGL; Receptor tyrosine-protein kinase erbB-2; Metastatic lymph node gene 19 protein; MLN 19; Proto-oncogene Neu; Proto-oncogene c-ErbB-2; Tyrosine kinase-type cell surface receptor HER2; p185erbB2; CD antigen CD340
UNIPROT ID:   P04626
Background:    This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase.
Immunogen:    The antiserum was produced against synthesized peptide derived from human HER2 around the phosphorylation site of Tyr1221/Tyr1222. AA range:1191-1240
Applications:    WB,IHC-P,IP,ELISA
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100 IP: 1/20 ELISA: 1/10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Clone ID:   
MW:    Calculated MW: 138 kDa; Observed MW: 180 kDa
Isotype:    IgG
Purification:    Affinity Chromatography
Species Reactivity:    Human,Mouse,Rat
Conjugation:    Unconjugated
Modification:    Phosphorylated
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma, using HER2 (Phospho-Tyr1221/Tyr122, 2) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval. Sample with blocking peptide on the right.

Western blot analysis of Phospho-ErbB 2 (Tyr1221/Tyr1222) in various lysates using Phospho-ErbB 2 (Tyr1221/Tyr1222) antibody.

Western blot analysis of Phospho-ErbB 2 (Tyr1221/Tyr1222) in SKOV-3 lysates treated with EGF, using HER2 (Phospho-Tyr1221/Tyr122, 2) antibody. The lane on the right is blocked with the Phospho- peptide.
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