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Phospho-Histone H2A.X (Ser139) (7G9) Mouse mAb

Anti-Phospho-Histone H2A.X (Ser139) (7G9) Mouse Monoclonal Antibody
5/5

$349.00

Cat.#:  N261451

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   N261451
Product Name:   Anti-Phospho-Histone H2A.X (Ser139) (7G9) Mouse Monoclonal Antibody
Synonyms:   H2A.X; H2AFX; H2a/x; HIST5-2AX; Histone H2A.X; gamma H2A.X
UNIPROT ID:   P16104
Background:    Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
Immunogen:    Synthetic phosphopeptide corresponding to residues surrounding Ser139 of human H2A.X.
Applications:    WB,ICC/IF
Recommended Dilutions:    WB: 1/500-1/1000 IF: 1/50-1/200
Host Species:    Mouse
Clonality:    Mouse Monoclonal
Clone ID:    7G9-H4
MW:    Calculated MW: 15 kDa; Observed MW: 15 kDa
Isotype:    IgG1
Purification:    Affinity Purified
Species Reactivity:    Human,Mouse
Conjugation:    Unconjugated
Modification:    Phosphorylated
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    EpigeneticsyyyyyHistone phosphorylation
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunofluorescence analysis of Phospho-Histone H2A.X (Ser139) (7G9) in 3T3 or Hydroxyureatreated 3T3 using Phospho-Histone H2A.X (Ser139) antibody.

Western blot analysis of Phosphorylation of H2A.X at Serine 139 in 3T3 or Hydroxyureatreated 3T3 lysates using Phospho-Histone H2A.X (Ser139) antibody.

Immunofluorescence analysis of Phospho-Histone H2A.X (Ser139) (7G9) in A549(upper, untreated or Hydroxyureatreated) and Hela(lower, untreated or Hydroxyureatreated) using Phospho-Histone H2A.X (Ser139) antibody.
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