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Phospho-IKK beta (Tyr188) Rabbit pAb

Anti-Phospho-IKK beta (Tyr188) Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  N225486

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   N225486
Product Name:   Anti-Phospho-IKK beta (Tyr188) Rabbit pAb
Synonyms:   IKBKB; IKKB; Inhibitor of nuclear factor kappa-B kinase subunit beta; I-kappa-B-kinase beta; IKK-B; IKK-beta; IkBKB; I-kappa-B kinase 2; IKK2; Nuclear factor NF-kappa-B inhibitor kinase beta; NFKBIKB
UNIPROT ID:   O14920
Background:    The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins (1-3). Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB (3-7). The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits.
Immunogen:    The antiserum was produced against synthesized peptide derived from human IKK-beta around the phosphorylation site of Tyr188. AA range:161-210
Applications:    WB,IHC-P,ELISA
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100 ELISA: 1/10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Clone ID:   
MW:    Calculated MW: 87 kDa; Observed MW: 87 kDa
Isotype:    IgG
Purification:    Affinity Chromatography
Species Reactivity:    Human,Mouse,Rat,Monkey
Conjugation:    Unconjugated
Modification:    Phosphorylated
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

EnzymeLinked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phospho-peptide (Phospho-left) and NonPhospho-peptide (Phospho-right), using IKKbeta (Phospho-Tyr18antibody

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Phospho-IKK beta (Tyr188) antibody.High-pressure and temperature Tris-EDTA pH 8.0 was used for antigen retrieval.Sample with blocking peptide on the right.

Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma using Phospho-IKK beta (Tyr188) antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Sample with blocking peptide on the right.

Western blot analysis of Phospho-IKK beta (Tyr188) in COS7 lysates using Phospho-IKK beta (Tyr188) antibody.The lane on the right is blocked with the synthesized peptide.
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