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Phospho-MCM2 (Ser27) Rabbit mAb

Anti-Phospho-MCM2 (Ser27) Rabbit Monoclonal Antibody
5/5

$349.00

Cat.#:  N263175

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   N263175
Product Name:   Anti-Phospho-MCM2 (Ser27) Rabbit Monoclonal Antibody
Synonyms:   MCM2; BM28; CCNL1; CDCL1; KIAA0030; DNA replication licensing factor MCM2; Minichromosome maintenance protein 2 homolog; Nuclear protein BM28
UNIPROT ID:   P49736
Background:    The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Oct 2012]
Immunogen:    A synthetic phosphopeptide corresponding to residues surrounding Ser27 of human MCM2
Applications:    WB,IHC-F,IHC-P,ICC/IF,IP
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20
Host Species:    Rabbit
Clonality:    Rabbit Monoclonal
Clone ID:    R08-4D8
MW:    Calculated MW: 102 kDa; Observed MW: 125 kDa
Isotype:    IgG
Purification:    Affinity Purified
Species Reactivity:    Human
Conjugation:    Unconjugated
Modification:    Phosphorylated
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin-embedded Human tonsil using MCM2 (Phospho-Ser27) antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Immunocytochemistry analysis of Phospho-MCM2 (Ser27) (green) in Hela using Phospho-MCM2 (Ser27) antibody,and DAPI(blue).

Western blot analysis of MCM2 (Phospho-Ser27) in Hela, A549, HL-60, U2OS lysates using MCM2 (Phospho-Ser27) antibody.
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