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Phospho-PERK (Thr982) Rabbit pAb

Anti-Phospho-PERK (Thr982) Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  N225398

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   N225398
Product Name:   Anti-Phospho-PERK (Thr982) Rabbit pAb
Synonyms:   EIF2AK3; PEK; PERK; Eukaryotic translation initiation factor 2-alpha kinase 3; PRKR-like endoplasmic reticulum kinase; Pancreatic eIF2-alpha kinase; HsPEK
UNIPROT ID:   Q9NZJ5
Background:    Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (eIF-2-alpha/EIF2S1) on ‘Ser-52’ during the unfolded protein response (UPR) and in response to low amino acid availability. Converts phosphorylated eIF-2-alpha/EIF2S1 either in a global protein synthesis inhibitor, leading to a reduced overall utilization of amino acids, or to a translation initiation activator of specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1). Involved in control of mitochondrial morphology and function.
Immunogen:    The antiserum was produced against synthesized peptide derived from human PEK/PERK around the phosphorylation site of Thr982. AA range:947-996
Applications:    ICC/IF,WB,IHC-F,IHC-P,ELISA
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 ELISA: 1/10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Clone ID:   
MW:    Calculated MW: 125 kDa; Observed MW: 130 kDa
Isotype:    IgG
Purification:    Affinity Purified
Species Reactivity:    Human,Mouse,Rat
Conjugation:    Unconjugated
Modification:    Phosphorylated
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin-embedded mouse kidney tissue using Phospho-PERK (Thr982) antibody High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Western blot analysis of Phospho-PERK (Thr982) in A453, AD293, Hela lysates using Phospho-PERK (Thr982) antibody.

Immunohistochemistry analysis of paraffin-embedded rat lung tissue using Phospho-PERK (Thr982) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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