NewEast Biosciences pioneered the research and development of the antibodies for GTPases and mutated Oncogene ten years ago. GTPases involve (1) signal transduction in response to activation of cell surface receptors, including transmembrane receptors such as those mediating taste, smell and vision, (2) protein biosynthesis at the ribosome, (3) regulation of cell differentiation, proliferation, division and movement, (4) translocation of proteins through membranes, (5) transport of vesicles within the cell, and vesicle-mediated secretion and uptake, through GTPase control of vesicle coat assembly. An oncogene is a gene that has the potential to cause cancer.
We offer three unique categories of antibodies, which (1) recognize only the active configuration of GTPase (not the inactive one), (2) mutated Oncogene (not mild type) and (3) have super affinity for cAMP and cGMP (no acetylation required). We have over one thousand peer reviewed articles cited our products.
$349.00
Cat.#: N225740 |
Product Name: Anti-Phospho-Raf1 (Ser259) Rabbit pAb |
Synonyms: RAF1; RAF; RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF; cRaf; Raf-1 |
UNIPROT ID: P04049 |
Background: Raf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. |
Immunogen: A synthesized peptide derived from human Phospho-Raf1 (S259) |
Applications: WB,IHC-P |
Recommended Dilutions: WB: 1/500-1/1000 IHC: 1/50-1/100 |
Host Species: Rabbit |
Clonality: Rabbit Polyclonal |
Clone ID: – |
MW: Calculated MW: 73 kDa; Observed MW: 73 kDa |
Isotype: IgG |
Purification: Affinity Chromatography |
Species Reactivity: Human,Mouse,Rat |
Conjugation: Unconjugated |
Modification: Phosphorylated |
Constituents: PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide |
Research Areas: Signal Transduction |
Storage & Shipping: Store at -20°C. Avoid repeated freezing and thawing |
Western blot analysis of Phospho-Raf1 (S259) in (1) 293T lysates; (2) 293T lysates treated with Okadaic Acid + Calyculin A using Phospho-Raf1 (Ser259) antibody. |