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Phospho-Rb (Ser780) Rabbit pAb

Anti-Phospho-Rb (Ser780) Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  N225386

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   N225386
Product Name:   Anti-Phospho-Rb (Ser780) Rabbit pAb
Synonyms:   RB1; Retinoblastoma-associated protein; p105-Rb; pRb; Rb; pp110
UNIPROT ID:   P06400
Background:    Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression. Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation. Specificity of different CDK/cyclin complexes has been observed in vitro and cyclin D1 is required for Ser780 phosphorylation in vivo.
Immunogen:    The antiserum was produced against synthesized peptide derived from human Retinoblastoma around the phosphorylation site of Ser780. AA range:751-800
Applications:    WB,IHC-F,IHC-P,ICC/IF,ELISA
Recommended Dilutions:    WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 ELISA: 1/10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Clone ID:   
MW:    Calculated MW: 106 kDa; Observed MW: 106 kDa
Isotype:    IgG
Purification:    Affinity Chromatography
Species Reactivity:    Human,Mouse,Rat
Conjugation:    Unconjugated
Modification:    Phosphorylated
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma using Retinoblastoma (Phospho-Ser78) antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Sample with blocking peptide on the right.

Western blot analysis of Phospho-Rb (Ser780) in various lysates using Phospho-Rb (S780) antibody.

Western blot analysis of Phospho-Rb (Ser780) in KB lysates using Phospho-Rb (S780) antibody.

Western blot analysis of Phospho-Rb (Ser780) in K562 lysates treated with serum 10% using Phospho-Rb (Ser780) antibody.The lane on the right is blocked with the Phospho- peptide.
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