NewEast Biosciences pioneered the research and development of the antibodies for GTPases and mutated Oncogene ten years ago. GTPases involve (1) signal transduction in response to activation of cell surface receptors, including transmembrane receptors such as those mediating taste, smell and vision, (2) protein biosynthesis at the ribosome, (3) regulation of cell differentiation, proliferation, division and movement, (4) translocation of proteins through membranes, (5) transport of vesicles within the cell, and vesicle-mediated secretion and uptake, through GTPase control of vesicle coat assembly. An oncogene is a gene that has the potential to cause cancer.
We offer three unique categories of antibodies, which (1) recognize only the active configuration of GTPase (not the inactive one), (2) mutated Oncogene (not mild type) and (3) have super affinity for cAMP and cGMP (no acetylation required). We have over one thousand peer reviewed articles cited our products.
$349.00
Cat.#: N225623 |
Product Name: Anti-Phospho-Src (Tyr419) Rabbit pAb |
Synonyms: SRC; SRC1; Proto-oncogene tyrosine-protein kinase Src; Proto-oncogene c-Src; pp60c-src; p60-Src |
UNIPROT ID: P12931 |
Background: Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2). |
Immunogen: A synthesized peptide derived from human Phospho-Src (Y419) |
Applications: WB,FC |
Recommended Dilutions: WB: 1/500-1/1000 FC: 1/50-1/100 |
Host Species: Rabbit |
Clonality: Rabbit Polyclonal |
Clone ID: – |
MW: Calculated MW: 60 kDa; Observed MW: 60 kDa |
Isotype: IgG |
Purification: Affinity Chromatography |
Species Reactivity: Human,Mouse,Rat |
Conjugation: Unconjugated |
Modification: Phosphorylated |
Constituents: PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide |
Research Areas: Signal Transduction |
Storage & Shipping: Store at -20°C. Avoid repeated freezing and thawing |
Western blot analysis of Phospho-Src (Y419) in A431 lysates treated with pervanadate using Phospho-Src (Tyr419) antibody. |