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PIBF1 Rabbit pAb

Anti-PIBF1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S221636

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S221636
Product Name:   Anti-PIBF1 Rabbit Polyclonal Antibody
Synonyms:   PIBF; CEP90; C13orf24
UNIPROT ID:   Q8WXW3 (Gene Accession – NP_006337 )
Background:    PIBF (Progesterone-Induced Blocking Factor 1) is synthesized during pregnancy in response to progesterone by progesterone receptor-positive T lymphocytes (mostly gamma-delta T cells). In the presence of PIBF, natural killer (NK) cells inhibit the release of perforin from storage granules and therefore fail to lyse target cells. In humans, the amount of cells that express PIBF is significantly higher in healthy pregnant women than in women at risk for premature pregnancy termination. Full-length PIBF is associated with the nucleus, whereas secretion of shorter forms is induced by activation of the cell. Research suggests that PIBF functions as a transcription factor in its full-length form, while smaller forms may act as cytokines. The PIBF gene encodes a deduced hydrophilic 757-amino acid alpha-helical protein with an N-terminal signal sequence, a leucine zipper motif, a basic zipper sequence, a PEST sequence, a nuclear localization signal, an endoplasmic reticulum membrane retention signal, and many presumeed N-glycosylation and phosphorylation sites.
Immunogen:    Synthetic peptide of human PIBF1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: Oct-50;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Cell Biology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human prostate cancer tissue using 221636(PIBF1 Antibody) at a dilution of 1/25(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human prostate cancer tissue is first treated with the synthetic peptide and then with 221636(Anti-PIBF1 Antibody) at dilution 1/25.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-3: 231, K562 and 293T cell lysates;
Primary antibody: 221636(PIBF1 Antibody) at dilution 1/250;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 1 minute
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