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PIK3R2 Rabbit pAb

Anti-PIK3R2 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S220330

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S220330
Product Name:   Anti-PIK3R2 Rabbit Polyclonal Antibody
Synonyms:   p85; MPPH; P85B; p85-BETA
UNIPROT ID:   O00459 (Gene Accession – NP_005018 )
Background:    Phosphatidylinositol 3-kinase (PI3K) is a lipid kinase that phosphorylates phosphatidylinositol and similar compounds, creating second messengers important in growth signaling pathways. PI3K functions as a heterodimer of a regulatory and a catalytic subunit. The protein encoded by this gene is a regulatory component of PI3K. Two transcript variants, one protein coding and the other non-protein coding, have been found for this gene.
Immunogen:    Synthetic peptide of human PIK3R2
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-100;WB: 200-1000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Immunology
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human lymphoma tissue using 220330(PIK3R2 Antibody) at a dilution of 1/70(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human lymphoma tissue is first treated with the synthetic peptide and then with 220330(Anti-PIK3R2 Antibody) at dilution 1/70.

The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using 220330(Anti-PIK3R2 Antibody) at a dilution of 1/70.

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with synthetic peptide and then with D261372(Anti-PIK3R2 Antibody) at dilution 1/70.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-4: Raji cells, A549 cells, A431 cells, K562 cells;
Primary antibody: 220330(PIK3R2 Antibody) at dilution 1/233;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 2 minutes
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