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PINCH (3C12) Mouse mAb

Anti-PINCH (3C12) Mouse Monoclonal Antibody
5/5

$349.00

Cat.#:  N261198

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   N261198
Product Name:   Anti-PINCH (3C12) Mouse Monoclonal Antibody
Synonyms:   LIMS1; PINCH; PINCH1; LIM and senescent cell antigen-like-containing domain protein 1; Particularly interesting new Cys-His protein 1; PINCH-1; Renal carcinoma antigen NY-REN-48
UNIPROT ID:   P48059
Background:    The protein encoded by this gene is an adaptor protein which contains five LIM domains, or double zinc fingers. The protein is likely involved in integrin signaling through its LIM domain-mediated interaction with integrin-linked kinase, found in focal adhesion plaques. It is also thought to act as a bridge linking integrin-linked kinase to NCK adaptor protein 2, which is involved in growth factor receptor kinase signaling pathways. Its localization to the periphery of spreading cells also suggests that this protein may play a role in integrin-mediated cell adhesion or spreading. Several transcript variants encoding different isoforms have been found for this gene.
Immunogen:    Purified recombinant fragment of human PINCH expressed in E. Coli.
Applications:    WB,ICC/IF,FC,IP
Recommended Dilutions:    WB: 1/500-1/1000 IF: 1/50-1/200 IP: 1/20 FC: 1/50-1/100
Host Species:    Mouse
Clonality:    Mouse Monoclonal
Clone ID:    3C12-F7-A8
MW:    Calculated MW: 37 kDa; Observed MW: 37 kDa
Isotype:    IgG1
Purification:    Ascitic Fluid
Species Reactivity:    Human
Conjugation:    Unconjugated
Modification:    Unmodified
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Research Areas:    Cardiovascular
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunofluorescence analysis of PINCH (3C12) in HepG2 cells using PINCH (3C12) antibody (green)and DAPI (blue).

Immunoprecipitation analysis of PINCH in Hela lysates using PINCH antibody.

Western blot analysis of PINCH (3C12) in C6, Jurkat, K562, 3T3 and Hela lysates using PINCH (3C12) antibody

Flow cytometry analysis of Hela stained with PINCH antibody (blue) and negative control (red).
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