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PKMYT1 Rabbit pAb

Anti-PKMYT1 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S220812

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S220812
Product Name:   Anti-PKMYT1 Rabbit Polyclonal Antibody
Synonyms:   MYT1; PPP1R126
UNIPROT ID:   Q99640 (Gene Accession – NP_001245379 )
Background:    This gene encodes a member of the serine/threonine protein kinase family. The encoded protein is a membrane-associated kinase that negatively regulates the G2/M transition of the cell cycle by phosphorylating and inactivating cyclin-dependent kinase 1. The activity of the encoded protein is regulated by polo-like kinase 1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Immunogen:    Synthetic peptide of human PKMYT1
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human liver cancer tissue using 220812(PKMYT1 Antibody) at a dilution of 1/50(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human liver cancer tissue is first treated with the synthetic peptide and then with 220812(Anti-PKMYT1 Antibody) at dilution 1/50.

The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using 220812(Anti-PKMYT1 Antibody) at a dilution of 1/50.

In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer tissue is first treated with synthetic peptide and then with D262054(Anti-PKMYT1 Antibody) at dilution 1/50.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: Mouse heart tissue lysate;
Primary antibody: 220812(PKMYT1 Antibody) at dilution 1/800;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 20 seconds
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