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PLEKHG6 Rabbit pAb

Anti-PLEKHG6 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217624

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217624
Product Name:   Anti-PLEKHG6 Rabbit Polyclonal Antibody
Synonyms:   MyoGEF
UNIPROT ID:   Q3KR16 (Gene Accession – BC105961 )
Background:    PLEKHG6 functions as a guanine nucleotide exchange factor activating the small GTPase RHOA, which, in turn, induces myosin filament formation. It activates RHOG but not activate RAC1, or to a much lower extent than RHOA and RHOG. Part of a functional unit, involving MyoGEF, MYH10 and RHOA, at the cleavage furrow to advance furrow ingression during cytokinesis. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with EZR, required for normal macropinocytosis.
Immunogen:    Fusion protein of human PLEKHG6
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human lung cancer tissue using 217624(PLEKHG6 Antibody) at a dilution of 1/20(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human lung cancer tissue is first treated with the fusion protein and then with 217624(Anti-PLEKHG6 Antibody) at dilution 1/20.

The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using 217624(Anti-PLEKHG6 Antibody) at a dilution of 1/20.

In comparision with the IHC on the left, the same paraffin-embedded Human colon cancer tissue is first treated with fusion protein and then with D222720(Anti-PLEKHG6 Antibody) at dilution 1/20.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane: 293T cells;
Primary antibody: 217624(PLEKHG6 Antibody) at dilution 1/400;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 10 seconds
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