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PNPO Rabbit pAb

Anti-PNPO Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S221836

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S221836
Product Name:   Anti-PNPO Rabbit Polyclonal Antibody
Synonyms:   PDXPO; HEL-S-302
UNIPROT ID:   Q9NVS9 (Gene Accession – NP_060599 )
Background:    The enzyme encoded by this gene catalyzes the terminal, rate-limiting step in the synthesis of pyridoxal 5′-phosphate, also known as vitamin B6. Vitamin B6 is a required co-factor for enzymes involved in both homocysteine metabolism and synthesis of neurotransmitters such as catecholamine. Mutations in this gene result in pyridoxamine 5′-phosphate oxidase (PNPO) deficiency, a form of neonatal epileptic encephalopathy.
Immunogen:    Synthetic peptide of human PNPO
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 20-100;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism, Neuroscience
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human brain tissue using 221836(PNPO Antibody) at a dilution of 1/30(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human brain tissue is first treated with the synthetic peptide and then with 221836(Anti-PNPO Antibody) at dilution 1/30.

The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using 221836(Anti-PNPO Antibody) at a dilution of 1/30.

In comparision with the IHC on the left, the same paraffin-embedded Human gastric cancer tissue is first treated with synthetic peptide and then with D263598(Anti-PNPO Antibody) at dilution 1/30.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-4: Human fetal brain tissue, PC-3, Hela and 293T cell lysates;
Primary antibody: 221836(PNPO Antibody) at dilution 1/700;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 30 seconds
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