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POLD2 Rabbit pAb

Anti-POLD2 Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S219138

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S219138
Product Name:   Anti-POLD2 Rabbit Polyclonal Antibody
Synonyms:   
UNIPROT ID:   P49005 (Gene Accession – BC000459 )
Background:    This gene encodes the 50-kDa catalytic subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3′ to 5′ exonuclease activity and plays a critical role in DNA replication and repair. The encoded protein is required for the stimulation of DNA polymerase delta activity by the processivity cofactor proliferating cell nuclear antigen (PCNA). Expression of this gene may be a marker for ovarian carcinomas. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 5.
Immunogen:    Fusion protein of human POLD2
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-300;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human esophagus cancer tissue using 219138(POLD2 Antibody) at a dilution of 1/95(Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with the fusion protein and then with 219138(Anti-POLD2 Antibody) at dilution 1/95.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: HepG2 cell lysate;
Primary antibody: 219138(POLD2 Antibody) at dilution 1/1000;
Secondary antibody: HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution;
Exposure time: 30 seconds
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