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POLR3F Rabbit pAb

Anti-POLR3F Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S219053

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S219053
Product Name:   Anti-POLR3F Rabbit Polyclonal Antibody
Synonyms:   RPC6; RPC39
UNIPROT ID:   Q9H1D9 (Gene Accession – BC012588 )
Background:    The protein encoded by this gene is one of more than a dozen subunits forming eukaryotic RNA polymerase III (RNA Pol III), which transcribes 5S ribosomal RNA and tRNA genes. This protein has been shown to bind both TFIIIB90 and TBP, two subunits of RNA polymerase III transcription initiation factor IIIB (TFIIIB). Unlike most of the other RNA Pol III subunits, the encoded protein is unique to this polymerase. Alternative splicing results in multiple transcript variants.
Immunogen:    Fusion protein of human POLR3F
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-300;WB: 500-2000;ELISA: 5000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human esophagus cancer tissue using 219053(POLR3F Antibody) at a dilution of 1/50(Nucleus or Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human esophagus cancer tissue is first treated with the fusion protein and then with 219053(Anti-POLR3F Antibody) at dilution 1/50.

The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using 219053(Anti-POLR3F Antibody) at a dilution of 1/50.

In comparision with the IHC on the left, the same paraffin-embedded Human brain tissue is first treated with fusion protein and then with D225726(Anti-POLR3F Antibody) at dilution 1/50.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: PC-3 and HepG2 cell lysates;
Primary antibody: 219053(POLR3F Antibody) at dilution 1/500;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 25 seconds
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