Email: info@neweastbio.com   |  Call: 6109452007

PPAT Rabbit pAb

Anti-PPAT Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217482

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217482
Product Name:   Anti-PPAT Rabbit Polyclonal Antibody
Synonyms:   GPAT; PRAT; ATASE
UNIPROT ID:   Q06203 (Gene Accession – BC004200 )
Background:    The protein encoded by this gene is a member of the purine/pyrimidine phosphoribosyltransferase family. It is a regulatory allosteric enzyme that catalyzes the first step of de novo purine nucleotide biosythetic pathway. This gene and PAICS/AIRC gene, a bifunctional enzyme catalyzing steps six and seven of this pathway, are located in close proximity on chromosome 4, and divergently transcribed from an intergenic region.
Immunogen:    Fusion protein of human PPAT
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 100-300;WB: 500-2000;ELISA: 2000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism, Epigenetics and Nuclear Signaling
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human cervical cancer tissue using 217482(PPAT Antibody) at a dilution of 1/50(Cytoplasm and Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human cervical cancer tissue is first treated with the fusion protein and then with 217482(Anti-PPAT Antibody) at dilution 1/50.

The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using 217482(Anti-PPAT Antibody) at a dilution of 1/50.

In comparision with the IHC on the left, the same paraffin-embedded Human brain tissue is first treated with fusion protein and then with D222426(Anti-PPAT Antibody) at dilution 1/50.

Gel: 10%SDS-PAGE, Lysate: 40 μg;
Lane 1-5: Hela cells, Raji cells, Jurkat cells, A549 cells, NIH/3T3 cells;
Primary antibody: 217482(PPAT Antibody) at dilution 1/250;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 20 seconds
          Publications