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PPM1D Rabbit pAb

Anti-PPM1D Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S216877

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S216877
Product Name:   Anti-PPM1D Rabbit Polyclonal Antibody
Synonyms:   WIP1; PP2C-DELTA
UNIPROT ID:   O15297 (Gene Accession – BC033893 )
Background:    The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. The expression of this gene is induced in a p53-dependent manner in response to various environmental stresses. While being induced by tumor suppressor protein TP53/p53, this phosphatase negatively regulates the activity of p38 MAP kinase, MAPK/p38, through which it reduces the phosphorylation of p53, and in turn suppresses p53-mediated transcription and apoptosis. This phosphatase thus mediates a feedback regulation of p38-p53 signaling that contributes to growth inhibition and the suppression of stress induced apoptosis. This gene is located in a chromosomal region known to be amplified in breast cancer. The amplification of this gene has been detected in both breast cancer cell line and primary breast tumors, which suggests a role of this gene in cancer development.
Immunogen:    Fusion protein of human PPM1D
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 40-200;WB: 200-1000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using 216877(PPM1D Antibody) at a dilution of 1/50(Cytoplasm or Nucleus).

Immunohistochemistry analysis of paraffin-embedded Human esophagus cancer tissue using 216877(Anti-PPM1D Antibody) at a dilution of 1/50.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane: Human placenta tissue lysate;
Primary antibody: 216877(PPM1D Antibody) at dilution 1/200;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 30 seconds
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