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PPM1F Rabbit pAb

Anti-PPM1F Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S220828

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S220828
Product Name:   Anti-PPM1F Rabbit Polyclonal Antibody
Synonyms:   CAMKP; FEM-2; POPX2; hFEM-2; CaMKPase
UNIPROT ID:   P49593 (Gene Accession – NP_055449 )
Background:    The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase.
Immunogen:    Synthetic peptide of human PPM1F
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 500-2000;ELISA: 2000-5000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction, Cancer
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human thyroid cancer tissue using 220828(PPM1F Antibody) at a dilution of 1/40(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with the synthetic peptide and then with 220828(Anti-PPM1F Antibody) at dilution 1/40.

The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using 220828(Anti-PPM1F Antibody) at a dilution of 1/40.

In comparision with the IHC on the left, the same paraffin-embedded Human colon cancer tissue is first treated with synthetic peptide and then with D262075(Anti-PPM1F Antibody) at dilution 1/40.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: K562 cells, human fetal liver tissue;
Primary antibody: 220828(PPM1F Antibody) at dilution 1/200;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 10 seconds
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