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PPOX Rabbit pAb

Anti-PPOX Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S220829

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S220829
Product Name:   Anti-PPOX Rabbit Polyclonal Antibody
Synonyms:   VP; PPO; V290M
UNIPROT ID:   P50336 (Gene Accession – NP_000300 )
Background:    This gene encodes the penultimate enzyme of heme biosynthesis, which catalyzes the 6-electron oxidation of protoporphyrinogen IX to form protoporphyrin IX. Mutations in this gene cause variegate porphyria, an autosomal dominant disorder of heme metabolism resulting from a deficiency in protoporphyrinogen oxidase, an enzyme located on the inner mitochondrial membrane. Alternatively spliced transcript variants encoding the same protein have been identified.
Immunogen:    Synthetic peptide of human PPOX
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 50-200;WB: 1000-5000;ELISA: 2000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Metabolism
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human thyroid cancer tissue using 220829(PPOX Antibody) at a dilution of 1/40(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with the synthetic peptide and then with 220829(Anti-PPOX Antibody) at dilution 1/40.

The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using 220829(Anti-PPOX Antibody) at a dilution of 1/40.

In comparision with the IHC on the left, the same paraffin-embedded Human breast cancer tissue is first treated with synthetic peptide and then with D262076(Anti-PPOX Antibody) at dilution 1/40.

Gel: 8%SDS-PAGE, Lysate: 40 μg;
Lane 1-2: K562 cells, 293T cells;
Primary antibody: 220829(PPOX Antibody) at dilution 1/200;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 30 seconds
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