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PPP1R12A Rabbit pAb

Anti-PPP1R12A Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S217626

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S217626
Product Name:   Anti-PPP1R12A Rabbit Polyclonal Antibody
Synonyms:   MBS; M130; MYPT1
UNIPROT ID:   O14974 (Gene Accession – BC111752 )
Background:    Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC.
Immunogen:    Fusion protein of human PPP1R12A
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: 25-100;WB: 1000-5000;ELISA: 2000-10000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human, Mouse, Rat
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Signal Transduction
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human thyroid cancer tissue using 217626(PPP1R12A Antibody) at a dilution of 1/25(Cytoplasm).

In comparision with the IHC on the left, the same paraffin-embedded Human thyroid cancer tissue is first treated with the fusion protein and then with 217626(Anti-PPP1R12A Antibody) at dilution 1/25.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane 1-4: NIH/3T3 cells, A172 cells, hela cells, PC3 cells;
Primary antibody: 217626(PPP1R12A Antibody) at dilution 1/350;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 40 seconds
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