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PPP1R13B Rabbit pAb

Anti-PPP1R13B Rabbit Polyclonal Antibody
5/5

$349.00

Cat.#:  S214146

   Size:   100 μl

In Stock

          Product Description          
Cat.#:   S214146
Product Name:   Anti-PPP1R13B Rabbit Polyclonal Antibody
Synonyms:   p85; ASPP1; p53BP2-like
UNIPROT ID:   Q96KQ4 (Gene Accession – NP_056131 )
Background:    This gene encodes a member of the ASPP (apoptosis-stimulating protein of p53) family of p53 interacting proteins. The protein contains four ankyrin repeats and an SH3 domain involved in protein-protein interactions. ASPP proteins are required for the induction of apoptosis by p53-family proteins. They promote DNA binding and transactivation of p53-family proteins on the promoters of proapoptotic genes. Expression of this gene is regulated by the E2F transcription factor.
Immunogen:    Synthetic peptide of human PPP1R13B
Applications:    ELISA, WB, IHC
Recommended Dilutions:    IHC: Oct-50;WB: 200-1000;ELISA: 1000-2000
Host Species:    Rabbit
Clonality:    Rabbit Polyclonal
Isotype:    Immunogen-specific rabbit IgG
Purification:    Antigen affinity purification
Species Reactivity:    Human
Constituents:    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.05% Sodium Azide and 40% glycerol
Research Areas:    Cancer, Stem Cells
Storage & Shipping:    Store at -20°C. Avoid repeated freezing and thawing

Immunohistochemistry analysis of paraffin embedded Human prostate cancer tissue using 214146(PPP1R13B Antibody) at a dilution of 1/20(Cytoplasm and Nucleus).

In comparision with the IHC on the left, the same paraffin-embedded Human prostate cancer tissue is first treated with the synthetic peptide and then with 214146(Anti-PPP1R13B Antibody) at dilution 1/20.

Gel: 6%SDS-PAGE, Lysate: 40 μg;
Lane: HepG2 cell;
Primary antibody: 214146(PPP1R13B Antibody) at dilution 1/300;
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution;
Exposure time: 5 seconds
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